Complement fixation test virus

Its great value is its predictable activity in the presence of serologically reacting factors and its nonspecificity, that is, it is not like an antibody with a narrow range of reactions or increased concentration occurring in a host following immunization or infection.

Furthermore, it is easily destroyed by heating at temperatures that have no deleterious effect on antibodies. It is the nature of complement not to react with an antigen or an antibody alone but to enter into combination with antigen-antibody complexes. The lack of specificity of complement allows it to react with almost any antigen-antibody complex.

Therefore, in this test procedure, unless complement is fixed by the particular antigen and antibody system in question, it will remain free to react as an indicator, resulting in a "negative" test.

If on the other hand, complement is fixed by the antigen and antibody system under study, it is not free to react in the indicator system, resulting in a "positive" test.

Last updated on June 9th, Enzyme-linked immunosorbent assay ELISA is extremely sensitive test that is used to detect specific antibodies or antigens. News Ticker. Fixation of Complement. Complement Fixation Test in Microtiter Plate rows 1 and 2 exhibit complement fixation obtained with acute and convalescent-phase serum specimens, respectively. Serological test. About Acharya Tankeshwar Articles.

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